Antisense Technology, Part A, General Methods, Methods of by John N. Abelson, Melvin I. Simon, M. Ian Phillips

By John N. Abelson, Melvin I. Simon, M. Ian Phillips

Antisense know-how is the facility to control gene expression inside of mammalian cells supplying strong experimental techniques for the examine of gene functionality and gene rules. for instance, tools that inhibit gene expression allow stories which probe the conventional functionality of a selected product inside a mobile. Such technique can be utilized in lots of disciplines comparable to pharmacology, oncology, genetics, telephone biology, developmental biology, molecular biology, biochemistry, and neurosciences. This quantity could be a very vital device in biomedical-oriented learn. The seriously acclaimed laboratory common for greater than 40 years, Methods in Enzymology is among the so much hugely revered courses within the box of biochemistry. considering 1955, each one quantity has been eagerly awaited, often consulted, and praised through researchers and reviewers alike. Now with greater than three hundred volumes (all of them nonetheless in print), the sequence comprises a lot fabric nonetheless suitable today--truly a necessary ebook for researchers in all fields of lifestyles sciences.

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DOYLE AND ITZHAK OFEK VOLUME 254. Oncogene Techniques Edited by PETER K. VOGT AND INDER M. VERMA VOLUME 255. Small GTPases and Their Regulators (Part A: Ras Family) Edited by W. E. BALCH, CHANNING J. DER, AND ALAN HALL VOLUME 256. Small GTPases and Their Regulators (Part B: Rho Family) Edited by W. E. BALCH, CHANNING J. DER, AND ALAN HALL VOLUME 257. Small GTPases and Their Regulators (Part C: Proteins Involved in Transport) Edited by W. E. BALCH, CHANNING J. DER, AND ALAN HALL VOLUME 258. Redox-Active Amino Acids in Biology Edited by JUDITH P.

For example, ISIS 1082 hybrid25 Z. Dominski and R. Kole, Proc. Natl. Acad. Sci. A. 90, 8673 (1993). D. Hodges and S. T. Crooke, Mol. Pharmacol. 48, 905 (1995). 27 S. Agrawal, J. Goodchild, M. P. Civeira, A. H. Thornton, P. S. Sarin, and P. C. Zamecnik, Proc. Natl. Acad. Sci. A. 85, 7079 (1988). 28 M. Lemaitre, B. Bayard, and B. Lebleu, Proc. Natl. Acad. Sci. A. 84, 648 (1987). 29 A. Rosolen, L. Whitesell, N. Ikegaki, R. H. Kennett, and L. M. Neckers, Cancer Res. 50, 6316 (1990). 30 G. Vasanthakumar and N.

Acad. Sci. A. 95, 11538 (1998). 79 [1] PROGRESS IN ANTISENSE TECHNOLOGY 19 specific binding events, each of which may have different characteristics and effects. Nonspecific binding to a wide variety of proteins has been demonstrated. Exemplary of this type of binding is the interaction of phosphorothioate oligonucleotides with serum albumin. The affinity of such interactions is low. 83,84 Furthermore, in this study, no competition between phosphorothioate oligonucleotides and several drugs that bind to bovine serum albumin was observed.

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