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18,19,20,21,22 and 23 Despite relevant results in SERS-based nano-system generation, the performances of these tags are far from the optimal. A poor control of aggregation and probe deposition in the hotspots issues still remain there. 19,20,21,22 and 23 Moreover, the presence of SERS-active nanostructures can also be optimized via post-synthetic strategy,24,25,26,27 and 28 making dimerlike structures ideal in a wide range of tagging, sensing, and analysis applications. 1 MATERIALS The following reagents were purchased from the indicated suppliers and used without further purification: biphenyl-4,4’-dithiol (DBDT, Aldrich), silver nitrate (AgNO3, Aldrich), and sodium citrate (Na3C3H5O(COO)3, Aldrich), N,N’-dicyclohexylcarbodiimide (DCC, Alfa Aesar), and N-hydroxysuccinimide (NHS, Aldrich).
In this paper the IR spectra of Cu/C and Ni/C nanocomposites are discussed (Figs. 9), which find a wider application as the material modifiers. Further, the bands appearing in the spectra and having the largest relative area were evaluated. We can see the difference in the intensity and number of absorption bands in the range 1300–1460 cm−1, which confirms the different structures of composites. In the range 600–800 cm−1, the bands with a very weak intensity are seen, which can be referred to the oscillations of double bonds (π-electrons) coordinated with metals.
After rinsing with PBS and immersed then in a solution of blocking buffer, the slide was stored overnight between 0 and 4°C. Human α-Thr was dissolved in PBS at concentrations of 102, 103, 104, 105, and 106 fM. The antigen solution (20 μL) was then printed on the microarray and allowed to react for 2 h in a humidity chamber. The slides were then rinsed in PBS containing sodium dodecyl sulfate (SDS) and in nanopure water. 1% Tween 20. Antitag aliquots (20 μL) were deposited on the glass slide and allowed to bind to the surface for 2 h.