By P. Ainslie
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9. (A) A complete resting assessment of intracranial blood flow using TCD should include blood velocities of middle cerebral artery (MCA), posterior cerebral artery (PCA), anterior cerebral artery (ACA) and basilar artery. The probe(s) can then be fixed on the arteries of interest for the remainder of the protocol. Electrocardiogram, end-tidal gases, and beat-to-beat blood pressure should be measured concomitantly with cerebral blood velocity metrics throughout the experiment. (B) The neurovascular coupling protocol should consist of 2 minutes each of resting with eyes open and closed, followed by 5-10 cycles of 20 seconds eyes closed and 40 seconds of reading with concurrent measurement of MCA and PCA velocities.
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Ogoh S, Fadel PJ, Nissen P, Jans Ø, Selmer C, Secher NH & Raven PB (2003). Baroreflexmediated changes in cardiac output and vascular conductance in response to alterations in carotid sinus pressure during exercise in humans. J Physiol (Lond) 550, 317–324. Ogoh S, Fisher JP, Fadel PJ & Raven PB (2007). Increases in central blood volume modulate carotid baroreflex resetting during dynamic exercise in humans. J Physiol (Lond) 581, 405–418. Ogoh S, Tzeng YC, Lucas SJ, Galvin SD & Ainslie PN (2009).